Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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Info-enriched characterization of mobile populations. Morphological data from images can insert into the richness of flow cytometry data. As an example, the figure exhibits an otherwise conventional apoptosis assay applying Annexin V and PI, incorporating mobile imaging to characterize cells in Every single population to expose morphologically distinctive options. These insights could not are actually attained from multiplex staining alone.
As samples are obtained about the Attune CytPix Flow Cytometer, the superior-speed brightfield digicam captures and suppliers photos of detected occasions at a fee around six,000 photographs per second, with regards to the flow price and picture dimensions. For larger versatility, Attune Cytometric Computer software lets you modify the picture seize frequency as desired and choose a specific gate for image capture, if essential.
Blend acoustic focusing know-how with superior-parameter Assessment for flexible spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating with a superior-velocity brightfield camera for simultaneous significant throughput flow cytometry and significant resolution brightfield imaging.
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On top of that, it really is illuminating to determine the amount of doublets slip through traditional singlet gates, and great to verify the standing of unanticipated double-optimistic situations in my Assessment."
Pair your Attune flow cytometer using an autosampler for amplified workflow efficiency, and include robotic integration for optimum throughput.
Constant impression high-quality even at higher throughput Acoustic concentrating as well as a large-pace digicam Merge to image these T cells consistently at reduced or large flow prices. Quickly regulate emphasis and digicam settings to meet experimental necessities.
Early indications of this contamination experienced originally been dismissed as particles. Backgating to discover new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also allows you to use morphological options to find out interesting subpopulations that could not be evident from flow cytometry info by yourself.
Possibly most of all, these events contain cells of clearly diverse phenotypes which may lead to incorrect conclusions about double positive occasions (specifically in rare populations).
Sound waves permit precise mobile alignment, leading to Outstanding data good more info quality and ~10X faster sample flow charges.
We Beforehand shown the strength of imaging Car or truck-T/Ramos mobile interactions. Enable’s look at just the population of finest desire, the double constructive situations, to learn more. We are able to now use prolonged morphological parameters (listed here, circularity and skewness of intensity) to additional look at the characteristics of these populations and refine gating on these gatherings, escalating info robustness.
Leverage the strength of State-of-the-art technological innovation and innovation to speed up your discoveries. Have confidence in within the Attune Flow Cytometer loved ones to deliver the dependability, pace, and precision that established your work aside.
Versatile payment program from Agilent Fiscal Options enable you to receive the vital instrumentation you will need for instant job, or to mature your lab.
At the heart of each and every Attune Flow Cytometer lies our groundbreaking acoustic concentrating engineering, which makes use of audio waves to meticulously align cells inside the flow mobile. This modern strategy surpasses traditional hydrodynamic solutions, leading to more quickly processing speeds, Fantastic data good quality, and amplified sample throughput.
As the cell line experienced Earlier been analyzed around the Attune CytPix Flow Cytometer, the researchers went back to the pictures and were being in the position to doc the microbial an infection not less than five times earlier.
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Here, we demonstrate that by gating approaches applying picture-dependent quantitative parameters can distinguish interacting cells from coincident activities to a lot more accurately analyze interacting cells.